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IL-1RL2 Polyclonal antibody

IL-1RL2 Polyclonal Antibody for FC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25677-1-AP

Print datasheet

Synonyms

IL 1Rrp2, IL1R rp2, IL1RL2, IL-1RL2, IL1RRP2, interleukin 1 receptor like 2

验证数据展示

  • WB analysis of K-562 using 25677-1-AP

    WB analysis of K-562 using 25677-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25677-1-AP (IL1RL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • FC experiment of K-562 using 25677-1-AP

    FC experiment of K-562 using 25677-1-AP

    1X10^6 K-562 cells were stained with 0.2ug IL1RL2 antibody (25677-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inK-562 cells
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

The immunogen of 25677-1-AP is IL-1RL2 Fusion Protein expressed in E. coli.

Tested Applications FC, WB,ELISA
Cited ApplicationsFC
Tested Reactivity human
Cited Reactivityhuman
Immunogen IL-1RL2 fusion protein Ag21974 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 1 receptor-like 2
Synonyms IL 1Rrp2, IL1R rp2, IL1RL2, IL-1RL2, IL1RRP2, interleukin 1 receptor like 2
Calculated Molecular Weight 575 aa, 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC107064
Gene Symbol IL1RL2
Gene ID (NCBI) 8808
RRIDAB_2880189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB29
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IL-1RL2 antibody 25677-1-APDownload protocol
FC protocol for IL-1RL2 antibody 25677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Am J Pathol

IL-36γ Augments Ocular Angiogenesis by Promoting the Vascular Endothelial Growth Factor-Vascular Endothelial Growth Factor Receptor Axis

Authors - WonKyung J Cho
View Article