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验证数据展示

  • FC experiment of U-937 using 60319-1-Ig

    FC experiment of U-937 using 60319-1-Ig

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human IL-1F10 (60319-1-Ig, Clone:5B2F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of Recombinant protein using 60319-1-Ig

    WB analysis of Recombinant protein using 60319-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60319-1-Ig (IL1F10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IL-1F10 Monoclonal antibody

IL-1F10 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 60319-1-Ig

产品说明书

CloneNo. 5B2F5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

IL1F10, Family of interleukin 1-theta, FIL1 theta, FIL1T, FKSG75

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inRecombinant protein
Positive FC (Intra) detected inU-937 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60319-1-Ig targets IL-1F10 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17250

Product name: Recombinant human IL-1F10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-152 aa of BC103966

Sequence: MCSLPMARYYIIKYADQKALYTRDGQLLVGDPVADNCCAEKICTLPNRGLDRTKVPIFLGIQGGSRCLACVETEEGPSLQLEDVNIEELYKGGEEATRFTFFQSSSGSAFRLEAAAWPGWFLCGPAEPQQPVQLTKESEPSARTKFYFEQSW

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 1 family, member 10 (theta)
别名 IL1F10, Family of interleukin 1-theta, FIL1 theta, FIL1T, FKSG75
计算分子量 152 aa, 17 kDa
GenBank蛋白编号BC103966
基因名称 IL1F10
Gene ID (NCBI) 84639
RRIDAB_2881430
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8WWZ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.

实验方案

Product Specific Protocols
WB protocol for IL-1F10 antibody 60319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols