• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30587-1-AP

    WB analysis using 30587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30587-1-AP (IL18RAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 30587-1-AP

    IHC staining of mouse spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 30587-1-AP

    IHC staining of rat spleen using 30587-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL18RAP Polyclonal antibody

IL18RAP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30587-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Accessory protein like, Accessory protein-like, ACPL, CD218 antigen-like family member B, CD218b

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, RAW 264.7 cells, rat spleen tissue
Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30587-1-AP targets IL18RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IL18RAP fusion protein Ag30327 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 18 receptor accessory protein
别名 Accessory protein like, Accessory protein-like, ACPL, CD218 antigen-like family member B, CD218b
计算分子量 599 aa, 68 kDa
观测分子量60 kDa
GenBank蛋白编号BC069630
基因名称 IL18RAP
Gene ID (NCBI) 8807
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95256
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-18 receptor accessory protein (IL18RAP, also known as IL1R7 and CD218b) is a crucial component of the IL-18 receptor complex. It enhances the binding activity of IL-18 to its receptor and plays a significant role in IL-18 signaling (PMID: 24842757). IL18RAP is related to the human tumor microenvironment and promotes various immune cell infiltration (PMID: 37698530).

实验方案

Product Specific Protocols
WB protocol for IL18RAP antibody 30587-1-APDownload protocol
IHC protocol for IL18RAP antibody 30587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols