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验证数据展示

  • WB analysis using 14153-1-AP

    WB analysis using 14153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14153-1-AP (IL-18BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 14153-1-AP

    WB analysis of A549 using 14153-1-AP

    WB result of IL-18BP antibody (14153-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18BP transfected A549 cells.

  • Featured Product
  • KD/KO Validated

IL-18BP Polyclonal antibody

IL-18BP Polyclonal Antibody for WB, ELISA
Cat No. 14153-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, ELISA

别名

IL18BP, IL 18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14153-1-AP targets IL-18BP in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag5335

Product name: Recombinant human IL-18BP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 30-194 aa of BC044215

Sequence: ATPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 18 binding protein
别名 IL18BP, IL 18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein
计算分子量 194 aa, 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC044215
基因名称 IL-18BP
Gene ID (NCBI) 10068
RRIDAB_2918034
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95998
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL18BP, also named as adekinig-alfa, has 3 isoforms (Isoform A 21kd; B 12kd and D 17kd). Isoform A binds to IL-18 and inhibits its activity. IL18BP functions as an inhibitor of the early TH1 cytokine response. Highly glycosylated of IL18BP will migrate to be 40kd. A 58kd protein is IL18BP crosslinked IL18.

实验方案

Product Specific Protocols
WB protocol for IL-18BP antibody 14153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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