验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14153-1-AP targets IL-18BP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag5335 Product name: Recombinant human IL-18BP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-194 aa of BC044215 Sequence: ATPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQQG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 18 binding protein |
| 别名 | IL18BP, IL 18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein |
| 计算分子量 | 194 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC044215 |
| 基因名称 | IL-18BP |
| Gene ID (NCBI) | 10068 |
| RRID | AB_2918034 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95998 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL18BP, also named as adekinig-alfa, has 3 isoforms (Isoform A 21kd; B 12kd and D 17kd). Isoform A binds to IL-18 and inhibits its activity. IL18BP functions as an inhibitor of the early TH1 cytokine response. Highly glycosylated of IL18BP will migrate to be 40kd. A 58kd protein is IL18BP crosslinked IL18.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-18BP antibody 14153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
