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验证数据展示

  • FC experiment of Transfected HEK-293T using Biotin-FcA98206

    FC experiment of Transfected HEK-293T using Biotin-FcA98206

    1x10^6 untransfected (left) or IL-7-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Biotin Anti-Human IL-7 Rabbit RecAb (Biotin-FcA98206, Clone: 241000F3) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and PE Streptavidin (Cat.No. PF00021). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of Transfected HEK-293T using Biotin-FcA98206

    FC experiment of Transfected HEK-293T using Biotin-FcA98206

    1x10^6 untransfected (left) or IL-7-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Biotin Anti-Human IL-7 Rabbit RecAb (Biotin-FcA98206, Clone: 241000F3) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin (Cat.No. PF00021). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® Biotin Anti-Human IL-7 Rabbit Recombinant Antibody

IL-7 FcZero-rAb® Recombinant Antibody for FC (Intra), ELISA
Cat No. Biotin-FcA98206

产品说明书

CloneNo. 241000F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

IL7, IL 7, interleukin 7, Interleukin-7

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-FcA98206 targets IL-7 in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg0830

Product name: Recombinant Human IL-7 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-177 aa of NM_000880.4

Sequence: DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 7
别名 IL7, IL 7, interleukin 7, Interleukin-7
计算分子量20 kDa
GenBank蛋白编号NM_000880.4
基因名称 IL-7
Gene ID (NCBI) 3574
偶联类型 Biotin
最大激发/发射波长-
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13232-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

实验方案

Product Specific Protocols
FC protocol for Biotin IL-7 antibody Biotin-FcA98206Download protocol
Standard Protocols
Click here to view our Standard Protocols
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