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IL-6 Recombinant antibody

IL-6 Recombinant Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2E9

Cat No : 81077-1-RR

Print datasheet

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

验证数据展示

  • WB analysis of HUVEC using 81077-1-RR

    WB analysis of HUVEC using 81077-1-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-87 MG using 81077-1-RR

    WB analysis of U-87 MG using 81077-1-RR

    Protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 81077-1-RR (IL-6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHUVEC cells, Protein transport inhibitor treated U-87 MG cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81077-1-RR targets IL-6 as part of a matched antibody pair:

MP80002-1: 81077-1-RR capture and 68659-10-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB,ELISA
Tested Reactivity Human
Immunogen IL-6 fusion protein Ag10565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 6 (interferon, beta 2)
Synonyms B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6
Calculated Molecular Weight 212 aa, 24 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC015511
Gene Symbol IL6
Gene ID (NCBI) 3569
ENSEMBL Gene IDENSG00000136244
RRIDAB_2918927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05231
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IL-6 antibody 81077-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols