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验证数据展示

  • WB analysis using 30953-1-AP

    WB analysis using 30953-1-AP

    LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 30953-1-AP (IL-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IL-6 Polyclonal antibody

IL-6 Polyclonal Antibody for WB, ELISA
Cat No. 30953-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, ELISA

别名

Ifnb2, Il6, IL-6, ILg6, interleukin 6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLPS and Brefeldin A treated NR8383 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30953-1-AP targets IL-6 in WB, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 rat
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 6
别名 Ifnb2, Il6, IL-6, ILg6, interleukin 6
计算分子量24 kDa
观测分子量24 kDa
GenBank蛋白编号NM_012589.2
基因名称 Il6
Gene ID (NCBI) 24498
RRIDAB_3669793
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20607
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

实验方案

Product Specific Protocols
WB protocol for IL-6 antibody 30953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols