验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30416-1-AP targets IL-5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg0380 Product name: Recombinant Human IL-5 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 20-134 aa of BC066282 Sequence: IPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 5 (colony-stimulating factor, eosinophil) |
| 别名 | IL5, B-cell differentiation factor I, IL 5, Interleukin 5, Interleukin-5 |
| 计算分子量 | 134 aa, 15 kDa |
| GenBank蛋白编号 | BC066282 |
| 基因名称 | IL5 |
| Gene ID (NCBI) | 3567 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05113 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interleukin-5 (IL-5) is a pleiotropic cytokine primarily produced by activated T helper 2 (Th2) cells, mast cells, and group 2 innate lymphoid cells (ILC2s). It plays a critical role in the regulation of eosinophil proliferation, differentiation, and activation, making it a key mediator in allergic inflammation, parasitic infections, and certain autoimmune diseases.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-5 antibody 30416-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
