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验证数据展示

  • Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4

    Sandwich ELISA standard curve of MP01293-4, Rat IL-4 Recombinant Matched Antibody Pair - PBS only. 84422-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1113. 84422-5-PBS was Biotin conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    Serum of eight individual healthy human donors was measured. Seven measured less than the lowest rat IL-4 standard, 39.1 pg/mL. One sample read 18.7 pg/mL.

  • Sample test of MP01293-4

    Sample test of MP01293-4

    One rat thymus was cultured for 18 hours in RPMI 1640 supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, and 100 μg/mL of streptomycin sulfate. The cells were stimulated with 5 μg/mL of PHA and 50 ng/mL of PMA for 7 days. An aliquot of the cell culture supernatant was removed, assayed for rat IL-4 and measured 98.5 pg/mL.

  • Affinity and Kinetic Characterization of 84422-3-PBS

    Affinity and Kinetic Characterization of 84422-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 84422-3-PBS against Rat IL-4 were performed. The affinity constant is below 1 pM.

Rat IL-4 Recombinant antibody, PBS Only (Capture)

IL-4 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84422-3-PBS

产品说明书

CloneNo. 241249E11

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

Sandwich ELISA, Indirect ELISA, Sample test

别名

B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84422-3-PBS targets IL-4 as part of a matched antibody pair:

MP01293-4: 84422-3-PBS capture and 84422-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 rat
免疫原 IL-4 fusion protein Eg1113 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 4
别名 B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1, Il4
计算分子量16kDa
GenBank蛋白编号NM_201270.1
基因名称 IL-4
Gene ID (NCBI) 287287
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20096
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C