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验证数据展示

  • WB analysis using 83765-7-RR

    WB analysis using 83765-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83765-7-RR (IL-33 antibody) at dilution of 1:2200 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83765-7-RR

    IHC staining of human tonsillitis using 83765-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83765-7-RR (IL-33 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 83765-7-RR

    IHC staining of human tonsillitis using 83765-7-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83765-7-RR (IL-33 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity of 83765-7-RR

    Affinity of 83765-7-RR

    Biolayer interferometry (BLI) kinetic assays of 83765-7-RR against Human IL-33 were performed. The affinity constant is 0.468 nM.

IL-33 Recombinant monoclonal antibody

IL-33 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83765-7-RR

产品说明书

CloneNo. 252156C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

IL33, C9orf26, IL 1F11, IL 33, IL-1F11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, hTERT-RPE1 cells, HeLa cells, human artery, mouse liver tissue, untreated RAW 264.7 cells, PMA and LPS-treated RAW 264.7 cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4400
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83765-7-RR targets IL-33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg7061

Product name: Recombinant human IL33 protein

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 122-270 aa of BC047085

Sequence: YLASLSTYNDQSITFALEDESYEIYVEDLKKDEKKDKVLLSYYESQHPSNESGDGVDGKMLMVTLSPTKDFWLHANNKEHSVELHKCEKPLPDQAFFVLHNMHSNCVSFECKTDPGVFIGVKDNHLALIKVDSSENLCTENILFKLSET

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 33
别名 IL33, C9orf26, IL 1F11, IL 33, IL-1F11
计算分子量 30 kDa
观测分子量31 kDa, 18 kDa
GenBank蛋白编号BC047085
基因名称 IL-33
Gene ID (NCBI) 90865
RRIDAB_3743495
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95760
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-33 is an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. IL-33 is synthesized as a 30 kDa full-length precursor that can be processed into an ~18-20 kDa mature form by inflammatory proteases, with the mature form exhibiting substantially higher biological activity as an extracellular alarmin cytokine.

实验方案

Product Specific Protocols
IHC protocol for IL-33 antibody 83765-7-RRDownload protocol
WB protocol for IL-33 antibody 83765-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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