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验证数据展示

  • WB analysis using 31127-1-AP

    WB analysis using 31127-1-AP

    Mouse skin tissues were subjected to SDS PAGE followed by western blot with 31127-1-AP (IL-1RN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 31127-1-AP

    IF Staining of A431 using 31127-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RN antibody (31127-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

IL-1RA  Polyclonal antibody

IL-1RA  Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31127-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse skin tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31127-1-AP targets IL-1RA  in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 1 receptor antagonist
别名 IL-1RN, Il1rn, Interleukin-1, IL-1RA, IL 1ra
计算分子量18KD
观测分子量18 kDa
GenBank蛋白编号NM_031167.5
基因名称 IL-1RA 
Gene ID (NCBI) 16181
RRIDAB_3669864
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ542W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The interleukin-1 receptor antagonist (IL-1RN) belongs to the IL-1 family. L1RN is a protein that binds to IL1 receptors, and inhibits the binding of IL1-alpha and IL1-beta.

实验方案

Product Specific Protocols
WB protocol for IL-1RA  antibody 31127-1-APDownload protocol
IF protocol for IL-1RA  antibody 31127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols