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验证数据展示

  • WB analysis using 82907-1-RR

    WB analysis using 82907-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82907-1-RR (IL-18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82907-1-RR

    WB analysis of HeLa using 82907-1-RR

    WB result of IL-18 antibody (82907-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells.

  • IHC staining of human tonsillitis using 82907-1-RR

    IHC staining of human tonsillitis using 82907-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IL18 antibody included
  • IHC staining of human tonsillitis using 82907-1-RR

    IHC staining of human tonsillitis using 82907-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82907-1-RR (IL-18 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IL18 antibody included
  • FC experiment of HeLa using 82907-1-RR

    FC experiment of HeLa using 82907-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug IL-18 Recombinant antibody (82907-1-RR, Clone:1H14) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HeLa using 82907-1-RR

    IF Staining of HeLa using 82907-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (82907-1-RR, Clone: 1H14 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82907-1-RR

    Affinity and Kinetic Characterization of 82907-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82907-1-RR against Human IL-18 were performed. The affinity constant is 0.769 nM.

  • Featured Product
  • KD/KO验证

IL-18 Recombinant monoclonal antibody

IL-18 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 82907-1-RR

产品说明书

CloneNo. 1H14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

IL18, Pre-IL18, 1H14, Iboctadekin, IFN-gamma-inducing factor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, U2OS cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82907-1-RR targets IL-18 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1063

Product name: Recombinant human Pre IL-18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC007461

Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IL-18
别名 IL18, Pre-IL18, 1H14, Iboctadekin, IFN-gamma-inducing factor
计算分子量 193 aa, 22 kDa
观测分子量22 kDa
GenBank蛋白编号BC007461
基因名称 IL-18
Gene ID (NCBI) 3606
RRIDAB_3670636
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14116
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.

实验方案

Product Specific Protocols
FC protocol for IL-18 antibody 82907-1-RRDownload protocol
IF protocol for IL-18 antibody 82907-1-RRDownload protocol
IHC protocol for IL-18 antibody 82907-1-RRDownload protocol
WB protocol for IL-18 antibody 82907-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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