IL-18 Recombinant antibody
IL-18 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1H14
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, U2OS cells, HeLa cells |
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy IL18 Ready-To-Use IHC Kit. IL18 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82907-1-RR targets IL-18 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA |
Tested Reactivity | Human |
Immunogen | IL-18 fusion protein Ag1063 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | IL-18 |
Synonyms | Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18 |
Calculated Molecular Weight | 193 aa, 22 kDa |
Observed Molecular Weight | 22 kDa |
GenBank Accession Number | BC007461 |
Gene Symbol | IL18 |
Gene ID (NCBI) | 3606 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-18 is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL-18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form.
实验方案
Product Specific Protocols | |
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WB protocol for IL-18 antibody 82907-1-RR | Download protocol |
IF protocol for IL-18 antibody 82907-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |