验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, EL-4 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30589-1-AP targets IL-17F in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 17F |
| 别名 | Il17f, interleukin 17F, IL 17F, C87042 |
| 计算分子量 | 17KD |
| 观测分子量 | 17-25 kDa |
| GenBank蛋白编号 | NM_145856.2 |
| 基因名称 | IL-17F |
| Gene ID (NCBI) | 257630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7TNI7-2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-17F, which is expressed by helper T cell 17(Th17) (at protein level). The expression pattern reflects the differentiation state. In fully differentiated Th17 cells, the level of IL-17a-IL-17f heterodimers is higher than that of IL-17a-IL-17a and IL-17f-IL-17f dimers (PMID:18025225), which are mainly secreted in the intestine (PMID:29915298). It is expressed by resident cells, epithelial cells and immune cell subsets of lamina propria (including natural killer cells, dendritic cells, macrophages and various T and B cell subsets) (PMID:16990136, PMID:29915298). The molecular weight of IL-17F is 17 kDa. IL-17F has the strongest similarity with IL-17A, and forms homodimer or heterodimer with IL-17A.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-17F antibody 30589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |