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验证数据展示

  • WB analysis using 85754-4-RR (same clone as 85754-4-PBS)

    WB analysis using 85754-4-RR (same clone as 85754-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85754-4-RR (IL-12A/IL-12 p35 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85754-4-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 85754-4-RR (same clone as 85754-4-PBS)

    IF Staining of THP-1 using 85754-4-RR (same clone as 85754-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN gamma and LPS treated THP-1 cells using IL-12A/IL-12 p35 antibody (85754-4-RR, Clone: 242887G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85754-4-PBS in a different storage buffer formulation.

IL-12A/IL-12 p35 Recombinant monoclonal antibody, PBS Only

IL-12A/IL-12 p35 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85754-4-PBS

产品说明书

CloneNo. 242887G5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

IL-12, CLMF, CLMF p35, IL 12 subunit p35, IL 12A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85754-4-PBS targets IL-12A/IL-12 p35 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3341

Product name: Recombinant Human IL12A protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-219 aa of BC104982

Sequence: RNLPVATPDPGMFPCLHHSQNLLRAVSNMLQKARQTLEFYPCTSEEIDHEDITKDKTSTVEACLPLELTKNESCLNSRETSFITNGSCLASRKTSFMMALCLSSIYEDLKMYQVEFKTMNAKLLMDPKRQIFLDQNMLAVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
别名 IL-12, CLMF, CLMF p35, IL 12 subunit p35, IL 12A
计算分子量 25 kDa
观测分子量65 kDa
GenBank蛋白编号BC104982
基因名称 IL-12A
Gene ID (NCBI) 3592
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP29459
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-12A (Interleukin-12A) is a subunit of the cytokine IL-12, which is also known as IL-12 p35. IL-12 is a heterodimeric cytokine composed of two subunits: IL-12A (p35) and IL-12B (p40). This cytokine plays a crucial role in the immune response, particularly in the activation and differentiation of T cells and natural killer (NK) cells. This antibody recognizes IL-12A and IL-12 P70, which consists of IL-12A and B, but does not recognize IL-12B.

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