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验证数据展示

  • FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were surface stained with CoraLite ® Plus 750 Anti-Human HLA-DR then intracellularly stained with 0.2 ug CoraLite ® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) or Mouse IgG1 Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    FC experiment of human PBMCs using 65277-1-Ig (same clone as 65277-1-PBS)

    1X10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (red) or Mouse IgG1 Isotype Control (blue), or unstimulated and Brefeldin A treated human PBMCs were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IL-12 (CL488-65277, Clone:1E12H6) (black, dashed line). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated. This data was developed using the CoraLite ® Plus 488 conjugated antibody of 65277-1-Ig. This data was developed using the same antibody clone with 65277-1-PBS in a different storage buffer formulation.

Anti-Human IL-12 p35 (1E12H6), PBS Only

IL-12 p35 Monoclonal Antibody for FC (Intra)
Cat No. 65277-1-PBS

产品说明书

CloneNo. 1E12H6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

65277-1-PBS targets IL-12 p35 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 IL-12 p35 fusion protein HZ-1256 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
别名 IL-12, 1E12H6, CLMF, CLMF p35, IL 12 subunit p35
计算分子量 25 kDa
GenBank蛋白编号BC104982
基因名称 IL-12A
Gene ID (NCBI) 3592
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only, pH 7.3.
储存条件-20°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells (PMID: 24821971; 7612223).