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验证数据展示

  • WB analysis of Jurkat using 24780-1-AP

    WB analysis of Jurkat using 24780-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 24780-1-AP

    IP experiment of Jurkat using 24780-1-AP

    IP result of anti-IKZF5 (IP:24780-1-AP, 4ug; Detection:24780-1-AP 1:300) with Jurkat cells lysate 2800ug.

IKZF5 Polyclonal antibody

IKZF5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24780-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

IKZF5, PEGASUS, Zinc finger protein Pegasus, ZNFN1A5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IP detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy IKZF5 Ready-To-Use IHC Kit. IKZF5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24780-1-AP targets IKZF5 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20405

Product name: Recombinant human IKZF5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 140-376 aa of BC022564

Sequence: CELCSFRCSDRSNLSHHRRRKHKMVPIKGTRSSLSSKKMWGVLQKKTSNLGYSRRALINLSPPSVVVQKPDYLNDFTHEIPNIQTDSYESMAKTTPTGGLPRDPQELMVDNPLNQLSTLAGQLSSLPPENQNPASPDVVPCPDEKPFMIQQPSTQAVVSAVSASIPQSSSPTSPEPRPSHSQRNYSPVAGPSSEPSAHTSTPSIGNSQPSTPAPALPVQDPQLLHHCQHCDMYFADN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IKAROS family zinc finger 5 (Pegasus)
别名 IKZF5, PEGASUS, Zinc finger protein Pegasus, ZNFN1A5
计算分子量 420 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC022564
基因名称 IKZF5
Gene ID (NCBI) 64376
RRIDAB_2879717
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H5V7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IKZF5 antibody 24780-1-APDownload protocol
IP protocol for IKZF5 antibody 24780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols