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IKZF5 Polyclonal antibody

IKZF5 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24780-1-AP

Print datasheet

Synonyms

IKZF5, PEGASUS, Zinc finger protein Pegasus, ZNFN1A5

验证数据展示

  • WB analysis of Jurkat using 24780-1-AP

    WB analysis of Jurkat using 24780-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24780-1-AP (IKZF5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 24780-1-AP

    IP experiment of Jurkat using 24780-1-AP

    IP result of anti-IKZF5 (IP:24780-1-AP, 4ug; Detection:24780-1-AP 1:300) with Jurkat cells lysate 2800ug.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HL-60 cells
Positive IP detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy IKZF5 Ready-To-Use IHC Kit. IKZF5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24780-1-AP is IKZF5 Fusion Protein expressed in E. coli.

Tested Applications IP, WB,ELISA
Tested Reactivity human
Immunogen IKZF5 fusion protein Ag20405 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IKAROS family zinc finger 5 (Pegasus)
Synonyms IKZF5, PEGASUS, Zinc finger protein Pegasus, ZNFN1A5
Calculated Molecular Weight 420 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC022564
Gene Symbol IKZF5
Gene ID (NCBI) 64376
RRIDAB_2879717
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5V7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IKZF5 antibody 24780-1-APDownload protocol
IP protocol for IKZF5 antibody 24780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols