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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

    WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83471-5-RR

    Affinity and Kinetic Characterization of 83471-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

IKZF4 Recombinant antibody, PBS Only

IKZF4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83471-5-PBS

产品说明书

CloneNo. 240357D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83471-5-PBS targets IKZF4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IKZF4 fusion protein Ag34767 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IKAROS family zinc finger 4 (Eos)
别名 ZNFN1A4, Zinc finger protein Eos, KIAA1782, EOS, 240357D5
GenBank蛋白编号BC160169
基因名称 IKZF4
Gene ID (NCBI) 64375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H2S9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C