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验证数据展示

  • WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

    WB analysis using 83471-5-RR (same clone as 83471-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83471-5-RR (IKZF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    IF Staining of mouse stomach using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    IF Staining of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IKZF4 antibody (83471-5-RR, Clone: 240357D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    FC experiment of U2OS using 83471-5-RR (same clone as 83471-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug IKZF4 Recombinant antibody (83471-5-RR, Clone:240357D5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83471-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83471-5-RR

    Affinity and Kinetic Characterization of 83471-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83471-5-RR against Human IKZF4 were performed. The affinity constant is 21.3 nM.

IKZF4 Recombinant monoclonal antibody, PBS Only

IKZF4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83471-5-PBS

产品说明书

CloneNo. 240357D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IF-P, FC (Intra), ELISA

别名

240357D5, EOS, IKZF 4, KIAA1782, Zinc finger protein Eos

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83471-5-PBS targets IKZF4 in WB, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34767

Product name: Recombinant human IKZF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 268-457 aa of BC160169

Sequence: ERCHNYLQSLSTEAQALAGQPGDEIRDLEMVPDSMLHSSSERPTFIDRLANSLTKRKRSTPQKFVGEKQMRFSLSDLPYDVNSGGYEKDVELVAHHSLEPGFGSSLAFVGAEHLRPLRLPPTNCISELTPVISSVYTQMQPLPGRLELPGSREAGEGPEDLADGGPLLYRPRGPLTDPGASPSNGCQDST

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IKAROS family zinc finger 4 (Eos)
别名 240357D5, EOS, IKZF 4, KIAA1782, Zinc finger protein Eos
观测分子量60 kDa
GenBank蛋白编号BC160169
基因名称 IKZF4
Gene ID (NCBI) 64375
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9H2S9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)

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