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HELIOS Polyclonal antibody

HELIOS Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13554-1-AP

Print datasheet

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

验证数据展示

  • IF Staining of HeLa using 13554-1-AP

    IF Staining of HeLa using 13554-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13554-1-AP (HELIOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lymphoma using 13554-1-AP

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (HELIOS antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lymphoma using 13554-1-AP

    IHC staining of human lymphoma using 13554-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of Jurkat using 13554-1-AP

    WB analysis of Jurkat using 13554-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13554-1-AP

    IP experiment of Jurkat using 13554-1-AP

    IP result of anti-HELIOS (IP:13554-1-AP, 4ug; Detection:13554-1-AP 1:300) with Jurkat cells lysate 2400ug.

经过测试的应用

Positive WB detected inJurkat cells, human placenta tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13554-1-AP targets HELIOS in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HELIOS fusion protein Ag4357 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IKAROS family zinc finger 2 (Helios)
Synonyms HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC028936
Gene Symbol IKZF2
Gene ID (NCBI) 22807
RRIDAB_2124959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685).

实验方案

Product Specific Protocols
WB protocol for HELIOS antibody 13554-1-APDownload protocol
IHC protocol for HELIOS antibody 13554-1-APDownload protocol
IF protocol for HELIOS antibody 13554-1-APDownload protocol
IP protocol for HELIOS antibody 13554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Immunohorizons

Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells.

Authors - Bonnie Yen
View Article