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验证数据展示

  • WB analysis using 31833-1-AP (same clone as 31833-1-PBS)

    WB analysis using 31833-1-AP (same clone as 31833-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 31833-1-AP (same clone as 31833-1-PBS)

    IHC staining of mouse testis using 31833-1-AP (same clone as 31833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31833-1-AP (same clone as 31833-1-PBS)

    IF Staining of A431 using 31833-1-AP (same clone as 31833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31833-1-PBS in a different storage buffer formulation.

IK Polyclonal antibody, PBS Only

IK Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31833-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RED, Protein RER, Protein Red, IK factor, IK Cytokine

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31833-1-PBS targets IK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37095

Product name: Recombinant human IK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC013005

Sequence: MPERDSEPFSNPLAPDGHDVDDPHSFHQSKLTNEDFRKLLMTPRAAPTSAPPSKSRHHEMPREYNEDEDPAARRRKKKSYYAKLRQQEIERERELAEKYRDRAKERRDGVNKDYEETELISTTANYRAVGPTAEADKSAAEKRRQLIQESKFLGGDMEHTHLVKGLDFALLQKVRAEIASKEKEEEELMEKPQKETKKDEDPENKIEFKTRLGRNV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 IK cytokine, down-regulator of HLA II
别名 RED, Protein RER, Protein Red, IK factor, IK Cytokine
计算分子量 557 aa, 66 kDa
观测分子量70 kDa
GenBank蛋白编号BC013005
基因名称 IK
Gene ID (NCBI) 3550
RRIDAB_3670123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13123
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.

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