• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IGSF2 Monoclonal antibody

IGSF2 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

FC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1E6A7

Cat No : 66333-1-Ig

Print datasheet

Synonyms

CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7

验证数据展示

  • FC experiment of RAW 264.7 using 66333-1-Ig

    FC experiment of RAW 264.7 using 66333-1-Ig

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.2 ug Anti-Human IGSF2 (66333-1-Ig, Clone:1E6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of RAW 264.7 using 66333-1-Ig

    WB analysis of RAW 264.7 using 66333-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66333-1-Ig (IGSF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 66333-1-Ig

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 66333-1-Ig

    IHC staining of human lung cancer using 66333-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66333-1-Ig (IGSF2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66333-1-Ig targets IGSF2 in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IHC, WB,ELISA
Tested Reactivity human, mouse
Immunogen IGSF2 fusion protein Ag23297 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin superfamily, member 2
Synonyms CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC136484
Gene Symbol IGSF2
Gene ID (NCBI) 9398
RRIDAB_2881713
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ93033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2.

实验方案

Product Specific Protocols
WB protocol for IGSF2 antibody 66333-1-IgDownload protocol
IHC protocol for IGSF2 antibody 66333-1-IgDownload protocol
FC protocol for IGSF2 antibody 66333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols