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IGSF2 Polyclonal antibody

IGSF2 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26047-1-AP

Print datasheet

Synonyms

CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7

验证数据展示

  • WB analysis using 26047-1-AP

    WB analysis using 26047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26047-1-AP (IGSF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26047-1-AP

    IHC staining of human placenta using 26047-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26047-1-AP

    IHC staining of human placenta using 26047-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 26047-1-AP

    IF Staining of RAW 264.7 using 26047-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 26047-1-AP (IGSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of RAW 264.7 using 26047-1-AP

    FC experiment of RAW 264.7 using 26047-1-AP

    1X10^6 RAW 264.7 cells were stained with 0.2ug IGSF2 antibody (26047-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inHCT 116 cells, RAW 264.7 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 26047-1-AP is IGSF2 Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen IGSF2 fusion protein Ag23297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin superfamily, member 2
Synonyms CD101, Cell surface glycoprotein V7, EWI 101, EWI101, IGSF2, V7
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC136484
Gene Symbol IGSF2
Gene ID (NCBI) 9398
RRIDAB_2880350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93033
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2. This antibody specifically recognises the 115 kDa protein.

实验方案

Product Specific Protocols
WB protocol for IGSF2 antibody 26047-1-APDownload protocol
IHC protocol for IGSF2 antibody 26047-1-APDownload protocol
IF protocol for IGSF2 antibody 26047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

Identification of CD101 in Glioma: A Novel Prognostic Indicator Expressed on M2 Macrophages.

Authors - Yuyang Liu
View Article