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验证数据展示

  • WB analysis using 26047-1-AP

    WB analysis using 26047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26047-1-AP (IGSF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 26047-1-AP

    IHC staining of human placenta using 26047-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 26047-1-AP

    IHC staining of human placenta using 26047-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26047-1-AP (IGSF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of RAW 264.7 using 26047-1-AP

    IF Staining of RAW 264.7 using 26047-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 26047-1-AP (IGSF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IGSF2/CD101 Polyclonal antibody

IGSF2/CD101 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26047-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, RAW 264.7 cells, Jurkat cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

26047-1-AP targets IGSF2/CD101 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag23297

Product name: Recombinant human IGSF2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 260-603 aa of BC136484

Sequence: ETWMFITKKQTDQTTLRIQPAVKDFQVNITADSLFAEGKPLELVCLVVSSGRDPQLQGIWFFNGTEIAHIDAGGVLGLKNDYKERASQGELQVSKLGPKAFSLKIFSLGPEDEGAYRCVVAEVMKTRTGSWQVLQRKQSPDSHVHLRKPAARSVVVSTKNKQQVVWEGETLAFLCKAGGAESPLSVSWWHIPRDQTQPEFVAGMGQDGIVQLGASYGVPSYHGNTRLEKMDWATFQLEITFTAITDSGTYECRVSEKSRNQARDLSWTQKISVTVKSLESSLQVSLMSRQPQVMLTNTFDLSCVVRAGYSDLKVPLTVTWQFQPASSHIFHQLIRITHNGTIEW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin superfamily, member 2
别名 IGSF2, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101, EWI101, EWI 101
观测分子量 115 kDa
GenBank蛋白编号BC136484
基因名称 IGSF2
Gene ID (NCBI) 9398
RRIDAB_2880350
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93033
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGSF2, also known as CD101, is a low abundance protein. Expression of CD101 is found on monocytes, granulocytes and dendritic cells. In addition expression on T lymphocytes is important for cell activation. It plays a role as inhibitor of T-cells proliferation induced by CD3 and inhibits expression of IL2RA on activated T-cells and secretion of IL2. This antibody specifically recognises the 115 kDa protein.

实验方案

Product Specific Protocols
WB protocol for IGSF2/CD101 antibody 26047-1-APDownload protocol
IHC protocol for IGSF2/CD101 antibody 26047-1-APDownload protocol
IF protocol for IGSF2/CD101 antibody 26047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Immunol

Identification of CD101 in Glioma: A Novel Prognostic Indicator Expressed on M2 Macrophages.

Authors - Yuyang Liu
View Article