验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, A549 cells, K-562 cells, mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
11781-1-AP targets IgG Kappa Light Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2406 Product name: Recombinant human IGKV1-5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-239 aa of BC030814 Sequence: MRLPAQLLGLLMLWVSGSSGDIVMTQSPLSLPVTPGEPASISCRSSQSLLHSDGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISKVEAEDVGIYYCMQGLQTPQTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNTLQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin kappa variable 1-5 |
| 别名 | IGKV15, IGKV, IGKV1 5, IGKV1-5, L12 |
| 计算分子量 | 239 aa, 26 kDa |
| 观测分子量 | 26 kDa, 13 kDa |
| GenBank蛋白编号 | BC030814 |
| 基因名称 | IgG Kappa Light Chain |
| Gene ID (NCBI) | 28299 |
| RRID | AB_10953529 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody detects the kappa light chain of human IgG.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for IgG Kappa Light Chain antibody 11781-1-AP | Download protocol |
| IHC protocol for IgG Kappa Light Chain antibody 11781-1-AP | Download protocol |
| WB protocol for IgG Kappa Light Chain antibody 11781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
