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验证数据展示

  • IHC staining of human tonsillitis using 11781-1-AP

    IHC staining of human tonsillitis using 11781-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11781-1-AP (IgG Kappa Light Chain antibody) at dilution of 1:1000 (under 4x lens). heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 11781-1-AP

    WB analysis of human plasma using 11781-1-AP

    human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 11781-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11781-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma tissue, A549 cells, K-562 cells, mouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11781-1-AP targets IgG Kappa Light Chain in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
免疫原

CatNo: Ag2406

Product name: Recombinant human IGKV1-5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC030814

Sequence: MRLPAQLLGLLMLWVSGSSGDIVMTQSPLSLPVTPGEPASISCRSSQSLLHSDGYNYLDWYLQKPGQSPQLLIYLGSNRASGVPDRFSGSGSGTDFTLKISKVEAEDVGIYYCMQGLQTPQTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNTLQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin kappa variable 1-5
别名 IgG Kappa Light Chain, IGKV, IGKV1 5, IGKV15, IGKV1-5, L12, L12a, V1
计算分子量 239 aa, 26 kDa
观测分子量 26 kDa, 13 kDa
GenBank蛋白编号BC030814
基因名称 IgG Kappa Light Chain
Gene ID (NCBI) 28299
RRIDAB_10953529
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the kappa light chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
IHC protocol for IgG Kappa Light Chain antibody 11781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Arthritis Res Ther

Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/β-catenin pathway

Authors - Dong Guo
View Article