验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human liver tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14678-1-AP targets Ig Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6389 Product name: Recombinant human IGK- protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC030813 Sequence: MEAPAQLLFLLLLWLPDTTGEIVMTQSPATLSVSPGERATLSCRASQSVTSNLAWYQQTPGQSPRLVIYGASSRASGVPARFSGSGSGTEFTLTISSLQSEDFAVYYCQQYNKWPHTFGQGTKLDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin kappa locus |
| 别名 | IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC030813 |
| 基因名称 | IgG Kappa Light Chain |
| Gene ID (NCBI) | 50802 |
| RRID | AB_1851235 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody detects the light chain (Kappa) of human immunoglobulin.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Ig Kappa Light Chain antibody 14678-1-AP | Download protocol |
| WB protocol for Ig Kappa Light Chain antibody 14678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Protein Cell Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging | ||
Nat Commun A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice. | ||
PLoS Genet Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis. | ||
J Bone Miner Metab Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes. | ||
Mol Med Rep Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling. | ||
J Exp Clin Cancer Res RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner. |
