验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, mouse spleen tissue, human plasma |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IGJ Ready-To-Use IHC Kit. IGJ primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
13688-1-AP targets IGJ in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, hamster |
| 免疫原 |
CatNo: Ag4559 Product name: Recombinant human IGJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC038982 Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
| 别名 | Joining chain of multimeric IgA and IgM, JCHAIN, JCH, Immunoglobulin J chain, IGCJ |
| 计算分子量 | 158 aa, 18 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC038982 |
| 基因名称 | IGJ |
| Gene ID (NCBI) | 3512 |
| RRID | AB_2877973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01591 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGJ antibody 13688-1-AP | Download protocol |
| IP protocol for IGJ antibody 13688-1-AP | Download protocol |
| WB protocol for IGJ antibody 13688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine The secretion of sIgA and dendritic cells activation in the intestinal of cyclophosphamide-induced immunosuppressed mice are regulated by Alhagi honey polysaccharides. | ||
Int Immunopharmacol Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer. | ||
J Biotechnol Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells. | ||
Plant Mol Biol Plant-derived secretory component gives protease-resistance to Shiga toxin 1-specific dimeric IgA. | ||
Oncol Lett Oridonin induces apoptosis in HGC-27 cells by activating the JNK signaling pathway. |
