验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
23945-1-AP targets IGHMBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21057 Product name: Recombinant human IGHMBP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 642-993 aa of BC105088 Sequence: YLDDIVPENYSHENSQGSSHAATKPQGPAASTRTGSQRQEGGQEAAAPARQGRKKPAGKSLASEAPSQPSLNGGSPEGVESQDGVDHFRAMIVEFMASKKMQLEFPPSLNSHDRLRVHQIAEEHGLRHDSSGEGKRRFITVSKRAPRPRAALGPPAGTGGPAPLQPVPPTPAQTEQPPREQRGPDQPDLRTLHLERLQRVRSAQGQPASKEQQASGQQKLPEKKKKKAKGHPATDLPTEEDFEALVSAAVKADNTCGFAKCTAGVTTLGQFCQLCSRRYCLSHHLPEIHGCGERARAHARQRISREGVLYAGSGTKNGSLDPAKRAQLQRRLDKKLSELSNQRTSRRKERGT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin mu binding protein 2 |
| 别名 | ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2 |
| 计算分子量 | 993 aa, 109 kDa |
| 观测分子量 | 109 kDa |
| GenBank蛋白编号 | BC105088 |
| 基因名称 | IGHMBP2 |
| Gene ID (NCBI) | 3508 |
| RRID | AB_2879371 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P38935 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| WB protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv IGHMBP2 deletion suppresses translation and activates the integrated stress response | ||
Life Sci Alliance IGHMBP2 deletion suppresses translation and activates the integrated stress response |
