验证数据展示
经过测试的应用
| Positive WB detected in | human saliva tissue, human saliva, human plasma |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy Human IgA Ready-To-Use IHC Kit. Human IgA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:160000-1:640000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
60099-1-Ig targets Human IgA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human |
| 文献引用反应性 | rat |
| 免疫原 | Human IgA fusion protein Ag7417 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | immunoglobulin heavy constant alpha 1 |
| 别名 | Human IgA Heavy Chain, IGHA1, 3A5A5, IGHA 1, Igh2 |
| 计算分子量 | 496 aa, 53 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC016369 |
| 基因名称 | Human IgA Heavy Chain |
| Gene ID (NCBI) | 3493 |
| RRID | AB_1557448 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| IHC protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| IF protocol for Human IgA antibody 60099-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. | ||
Eur J Pharmacol Inhibition of excessive autophagy alleviates renal injury and inflammation in a rat model of immunoglobulin A nephropathy | ||
Hepatol Commun Spatial mapping of the HCC landscape identifies unique intratumoral perivascular-immune neighborhoods |
