验证数据展示
经过测试的应用
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33339-1-AP targets IGFL4 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag37543 Product name: Recombinant human IGFL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-124 aa of NM_001002923 Sequence: EGVTDLRLWLCQPAPRCGEWTYNPLEQCCDDGVILDLNQTRLCGSSCTFWPCFQHCCLESLGSQNQTVVRFKVPGMKPDCKSSPITRICAQEYHPKSPVSRSDLI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IGF-like family member 4 |
| 别名 | Insulin growth factor-like family member 4 |
| 计算分子量 | 14 kDa |
| GenBank蛋白编号 | NM_001002923 |
| 基因名称 | IGFL4 |
| Gene ID (NCBI) | 444882 |
| RRID | AB_3742946 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6B9Z1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGFL (Insulin-like growth factor like family) encode a family of small secreted proteins in Homo sapiens and Mus musculus. This family consists of four genes and two pseudogenes (IGFL1-4 and IGFL1P1-2). IGFL1 is expressed in the ovary and spinal cord. IGFL2 is expressed in the cerebellum, heart, placenta, spleen, stomach, testis and thymus. IGFL3 and IGFL4 are expressed in the cerebellum. The IGFL4 protein binds to the insulin-like growth factor receptor (IGF-1R) and the insulin receptor (IR), thereby activating downstream signaling pathways such as the PI3K/AKT and MAPK pathway.(PMID: 16890402).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGFL4 antibody 33339-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
