验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells, SKOV-3 cells, THP-1 cells |
| Positive IP detected in | mouse appendix tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IGFBP7 Ready-To-Use IHC Kit. IGFBP7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19961-1-AP targets IGFBP7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | insulin-like growth factor binding protein 7 |
| 别名 | IBP7, IBP-7, IGF-binding protein 7, IGFBP-7, Insulin-like growth factor-binding protein 7 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | NM_001553 |
| 基因名称 | IGFBP7 |
| Gene ID (NCBI) | 3490 |
| RRID | AB_2878630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16270 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGFBP7, also named as MAC25, PSF, IBP-7 and TAF, binds IGF-I and IGF-II with a relatively low affinity. IGFBP7 stimulates prostacyclin (PGI2) production. IGFBP7 stimulates cell adhesion.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| IP protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| WB protocol for IGFBP7 antibody 19961-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol Mechanisms Underlying Increased TIMP2 and IGFBP7 Urinary Excretion in Experimental AKI. | ||
Cancer Lett IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis. | ||
J Cell Mol Med IGFBP7 regulates cell proliferation and migration through JAK/STAT pathway in gastric cancer and is regulated by DNA and RNA methylation | ||
Hum Genet MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract. | ||
Heliyon IGFBP7 promotes gastric cancer by facilitating epithelial-mesenchymal transition of gastric cells |
