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验证数据展示

  • WB analysis of U-87 MG using 67567-1-Ig (same clone as 67567-1-PBS)

    WB analysis of U-87 MG using 67567-1-Ig (same clone as 67567-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 67567-1-Ig (IGFBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

    IHC staining of human liver using 67567-1-Ig (same clone as 67567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67567-1-Ig (IGFBP6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

  • IF Staining of HaCaT using 67567-1-Ig (same clone as 67567-1-PBS)

    IF Staining of HaCaT using 67567-1-Ig (same clone as 67567-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using IGFBP6 antibody (67567-1-Ig, Clone: 1D12B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67567-1-PBS in a different storage buffer formulation.

IGFBP6 Monoclonal antibody, PBS Only

IGFBP6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67567-1-PBS

产品说明书

CloneNo. 1D12B3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

IGFBP 6, IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67567-1-PBS targets IGFBP6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 IGFBP6 fusion protein Ag30050 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 6
别名 IGFBP 6, IGF-binding protein 6, IGF binding protein 6, IBP-6, IBP6
计算分子量 25 kDa
观测分子量 30-33 kDa
GenBank蛋白编号BC011708
基因名称 IGFBP6
Gene ID (NCBI) 3489
RRIDAB_2882781
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24592
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-like growth factor (IGF) binding protein (IGFBP6), a 240 amino acid protein, contains an IGFBP N-terminal domain and a thyroglobulin type-1 domain. It modulates the activity of IGF and shows independent effects of IGF, such as growth inhibition and apoptosis. It can decrease the proliferation and survival of cancer cells such as lung cancer cells and naso-pharyngeal cancer cells. IGFBP-6 is distinctive for its 50-fold higher binding affinity for IGF-II over IGF-I and this specificity makes it an attractive potential therapeutic candidate for IGF-II-dependent pediatric malignancies such as rhabdomyosarcoma (RMS). In addition, it was found that IGFBP6 can promote the migration of RMS cells in an IGF-independent manner, and MAPK pathways were involved in this process. Further study reported that IGFBP6 is one of most highly expressed proteins in varicose vein tissues and is involved in the proliferation of vascular smooth muscle cells (VSMCs), which may provide insights into the underlying pathogenesis of varicose vein.