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验证数据展示

  • WB analysis using 68512-1-Ig

    WB analysis using 68512-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68512-1-Ig (IGFBP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68512-1-Ig

    IHC staining of mouse brain using 68512-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68512-1-Ig (IGFBP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IGFBP5 Monoclonal antibody

IGFBP5 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68512-1-Ig

产品说明书

CloneNo. 1C5E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, SKOV-3 cells, Saos-2 cells, U2OS cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68512-1-Ig targets IGFBP5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 IGFBP5 fusion protein Ag33797 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 5
别名 IBP 5, IBP5, IGF binding protein 5, IGFBP 5, IGFBP5
计算分子量 31 kDa
观测分子量30-33 kDa
GenBank蛋白编号BC011453
基因名称 IGFBP5
Gene ID (NCBI) 3488
RRIDAB_3085221
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP24593
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGFBP5, also named as IBP5, prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. It alter the interaction of IGFs with their cell surface receptors. This antibody is specific to IGFBP5.

实验方案

Product Specific Protocols
WB protocol for IGFBP5 antibody 68512-1-IgDownload protocol
IHC protocol for IGFBP5 antibody 68512-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols