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验证数据展示

  • IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

    IHC staining of human breast cancer using 10189-2-AP (same clone as 10189-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IGFBP3 antibody included
  • WB analysis using 10189-2-AP (same clone as 10189-2-PBS)

    WB analysis using 10189-2-AP (same clone as 10189-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

    IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

    IF Staining of HeLa using 10189-2-AP (same clone as 10189-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10189-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IGFBP3 Polyclonal antibody, PBS Only

IGFBP3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10189-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IGFBP3 Ready-To-Use IHC Kit. IGFBP3 primary antibody included.

产品信息

10189-2-PBS targets IGFBP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0242

Product name: Recombinant human IGFBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 134-291 aa of BC000013

Sequence: PGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 insulin-like growth factor binding protein 3
别名 BP 53, IBP 3, IBP3, IBP-3, IGF binding protein 3
计算分子量 32 kDa
观测分子量 32 kDa, 40-45 kDa
GenBank蛋白编号BC000013
基因名称 IGFBP3
Gene ID (NCBI) 3486
RRIDAB_2123233
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17936
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

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