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验证数据展示

  • Sandwich ELISA sample test of MP02694-2

    Sandwich ELISA sample test of MP02694-2

    The mean IGF-II R/IGF2R concentration was determined to be 344.83 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load and 214.23 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA sample test of MP02694-2

    Sandwich ELISA sample test of MP02694-2

    Serum of four individual healthy human donors was measured. The human IGF-II R/IGF2R concentration of detected samples was determined to be 291.46 ng/mL with a range of 273.79 - 315.06 ng/mL.

  • Sandwich ELISA standard curve of MP02694-2

    Sandwich ELISA standard curve of MP02694-2

    Sandwich ELISA standard curve of MP02694-2, Human IGF-II R/IGF2R Recombinant Matched Antibody Pair - PBS only. 86821-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3488. 86821-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Cytometric bead array sample test of MP02694-1

    Cytometric bead array sample test of MP02694-1

    The mean IGF-II R/IGF2R concentration was determined to be 248.2 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 74.7 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02694-1

    Cytometric bead array standard curve of MP02694-1

    Cytometric bead array standard curve of MP02694-1, IGF-II R/IGF2R Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86821-3-PBS. Detection antibody: 86821-2-PBS. Standard: Eg3488. Range: 1.562-200 ng/mL.

  • Affinity and Kinetic Characterization of 86821-3-PBS

    Affinity and Kinetic Characterization of 86821-3-PBS

    Biolayer interferometry (BLl) kinetic assays of 86821-3-PBS against Human IGF-II R/IGF2R were performed. The affinity constant is 1.16 nM.

IGF-II R/IGF2R Recombinant monoclonal antibody, PBS Only (Capture)

IGF-II R/IGF2R Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86821-3-PBS

产品说明书

CloneNo. 251265C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

IGF2R, 300 kDa mannose 6-phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CI Man 6 P receptor

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IGF2R Ready-To-Use IHC Kit. IGF2R primary antibody included.

产品信息

86821-3-PBS targets IGF-II R/IGF2R as part of a matched antibody pair:

MP02694-1: 86821-3-PBS capture and 86821-2-PBS detection (validated in Cytometric bead array)

MP02694-2: 86821-3-PBS capture and 86821-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3488

Product name: recombinant human IGF-II R/IGF2R protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 41-1365 aa of NM_000876.4

Sequence: QAAPFPELCSYTWEAVDTKNNVLYKINICGSVDIVQCGPSSAVCMHDLKTRTYHSVGDSVLRSATRSLLEFNTTVSCDQQGTNHRVQSSIAFLCGKTLGTPEFVTATECVHYFEWRTTAACKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSERREGTIPKLTAKSNCRYEIEWITEYACHRDYLESKTCSLSGEQQDVSIDLTPLAQSGGSSYISDGKEYLFYLNVCGETEIQFCNKKQAAVCQVKKSDTSQVKAAGRYHNQTLRYSDGDLTLIYFGGDECSSGFQRMSVINFECNKTAGNDGKGTPVFTGEVDCTYFFTWDTEYACVKEKEDLLCGATDGKKRYDLSALVRHAEPEQNWEAVDGSQTETEKKHFFINICHRVLQEGKARGCPEDAAVCAVDKNGSKNLGKFISSPMKEKGNIQLSYSDGDDCGHGKKIKTNITLVCKPGDLESAPVLRTSGEGGCFYEFEWHTAAACVLSKTEGENCTVFDSQAGFSFDLSPLTKKNGAYKVETKKYDFYINVCGPVSVSPCQPDSGACQVAKSDEKTWNLGLSNAKLSYYDGMIQLNYRGGTPYNNERHTPRATLITFLCDRDAGVGFPEYQEEDNSTYNFRWYTSYACPEEPLECVVTDPSTLEQYDLSSLAKSEGGLGGNWYAMDNSGEHVTWRKYYINVCRPLNPVPGCNRYASACQMKYEKDQGSFTEVVSISNLGMAKTGPVVEDSGSLLLEYVNGSACTTSDGRQTTYTTRIHLVCSRGRLNSHPIFSLNWECVVSFLWNTEAACPIQTTTDTDQACSIRDPNSGFVFNLNPLNSSQGYNVSGIGKIFMFNVCGTMPVCGTILGKPASGCEAETQTEELKNWKPARPVGIEKSLQLSTEGFITLTYKGPLSAKGTADAFIVRFVCNDDVYSGPLKFLHQDIDSGQGIRNTYFEFETALACVPSPVDCQVTDLAGNEYDLTGLSTVRKPWTAVDTSVDGRKRTFYLSVCNPLPYIPGCQGSAVGSCLVSEGNSWNLGVVQMSPQAAANGSLSIMYVNGDKCGNQRFSTRITFECAQISGSPAFQLQDGCEYVFIWRTVEACPVVRVEGDNCEVKDPRHGNLYDLKPLGLNDTIVSAGEYTYYFRVCGKLSSDVCPTSDKSKVVSSCQEKREPQGFHKVAGLLTQKLTYENGLLKMNFTGGDTCHKVYQRSTAIFFYCDRGTQRPVFLKETSDCSYLFEWRTQYACPPFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 insulin-like growth factor 2 receptor
别名 IGF2R, 300 kDa mannose 6-phosphate receptor, Cation-independent mannose-6-phosphate receptor, CD222, CI Man 6 P receptor
计算分子量274 kDa
GenBank蛋白编号NM_000876.4
基因名称 IGF2R
Gene ID (NCBI) 3482
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11717
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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