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验证数据展示

  • WB analysis using 87726-1-RR

    WB analysis using 87726-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87726-1-RR (IGBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 87726-1-RR

    IP experiment of Jurkat using 87726-1-RR

    IP result of anti-IGBP1 (IP:87726-1-RR, 4ug; Detection:87726-1-RR 1:1000) with Jurkat cells lysate 1080 ug.

  • IF Staining of HeLa using 87726-1-RR

    IF Staining of HeLa using 87726-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IGBP1 antibody (87726-1-RR, Clone: 253170E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 87726-1-RR

    Affinity of 87726-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87726-1-RR against Human IGBP1 were performed. The affinity constant is 0.16 nM.

IGBP1 Recombinant monoclonal antibody

IGBP1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 87726-1-RR

产品说明书

CloneNo. 253170E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

ALPHA 4, B-cell signal transduction molecule alpha 4, CD79a binding protein 1, CD79a-binding protein 1, IBP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Raji cells, K-562 cells, Jurkat cells, Ramos cells, NIH/3T3 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87726-1-RR targets IGBP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6782

Product name: Recombinant human IGBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-339 aa of BC004137

Sequence: MAAEDELQLPRLPELFETGRQLLDEVEVATEPAGSRIVQEKVFKGLDLLEKAAEMLSQLDLFSRNEDLEEIASTDLKYLLVPAFQGALTMKQVNPSKRLDHLQRAREHFINYLTQCHCYHVAEFELPKTMNNSAENHTANSSMAYPSLVAMASQRQAKIQRYKQKKELEHRLSAMKSAVESGQADDERVREYYLLHLQRWIDISLEEIESIDQEIKILRERDSSREASTSNSSRQERPPVKPFILTRNMAQAKVFGAGYPSLPTMTVSDWYEQHRKYGALPDQGIAKAAPEEFRKAAQQQEEQEEKEEEDDEQTLHRAREWDDWKDTHPRGYGNRQNMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 immunoglobulin (CD79A) binding protein 1
别名 ALPHA 4, B-cell signal transduction molecule alpha 4, CD79a binding protein 1, CD79a-binding protein 1, IBP1
计算分子量 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC004137
基因名称 IGBP1
Gene ID (NCBI) 3476
RRIDAB_3745719
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

实验方案

Product Specific Protocols
IF protocol for IGBP1 antibody 87726-1-RRDownload protocol
IP protocol for IGBP1 antibody 87726-1-RRDownload protocol
WB protocol for IGBP1 antibody 87726-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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