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IGBP1 Polyclonal antibody

IGBP1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 14952-1-AP

Print datasheet

Synonyms

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

验证数据展示

  • WB analysis of Jurkat using 14952-1-AP

    WB analysis of Jurkat using 14952-1-AP

    WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

  • WB analysis of Raji using 14952-1-AP

    WB analysis of Raji using 14952-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14952-1-AP

    IP experiment of Jurkat using 14952-1-AP

    IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

经过测试的应用

Positive WB detected inRaji cells, Jurkat
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14952-1-AP targets IGBP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen IGBP1 fusion protein Ag6782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin (CD79A) binding protein 1
Synonyms ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC004137
Gene Symbol IGBP1
Gene ID (NCBI) 3476
RRIDAB_2121990
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

实验方案

Product Specific Protocols
WB protocol for IGBP1 antibody 14952-1-APDownload protocol
IP protocol for IGBP1 antibody 14952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols