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验证数据展示

  • WB analysis of Jurkat using 14952-1-AP

    WB analysis of Jurkat using 14952-1-AP

    WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

  • WB analysis using 14952-1-AP

    WB analysis using 14952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14952-1-AP

    IP experiment of Jurkat using 14952-1-AP

    IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

  • Featured Product
  • KD/KO验证

IGBP1 Polyclonal antibody

IGBP1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 14952-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

ALPHA 4, B-cell signal transduction molecule alpha 4, CD79a binding protein 1, CD79a-binding protein 1, IBP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, Raji cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14952-1-AP targets IGBP1 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6782

Product name: Recombinant human IGBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-339 aa of BC004137

Sequence: MAAEDELQLPRLPELFETGRQLLDEVEVATEPAGSRIVQEKVFKGLDLLEKAAEMLSQLDLFSRNEDLEEIASTDLKYLLVPAFQGALTMKQVNPSKRLDHLQRAREHFINYLTQCHCYHVAEFELPKTMNNSAENHTANSSMAYPSLVAMASQRQAKIQRYKQKKELEHRLSAMKSAVESGQADDERVREYYLLHLQRWIDISLEEIESIDQEIKILRERDSSREASTSNSSRQERPPVKPFILTRNMAQAKVFGAGYPSLPTMTVSDWYEQHRKYGALPDQGIAKAAPEEFRKAAQQQEEQEEKEEEDDEQTLHRAREWDDWKDTHPRGYGNRQNMG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 immunoglobulin (CD79A) binding protein 1
别名 ALPHA 4, B-cell signal transduction molecule alpha 4, CD79a binding protein 1, CD79a-binding protein 1, IBP1
计算分子量 39 kDa
观测分子量 40 kDa
GenBank蛋白编号BC004137
基因名称 IGBP1
Gene ID (NCBI) 3476
RRIDAB_2121990
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.

实验方案

Product Specific Protocols
IP protocol for IGBP1 antibody 14952-1-APDownload protocol
WB protocol for IGBP1 antibody 14952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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