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IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, Canine and More (4)


WB, IP, IF/ICC, IHC, ELISA and More (1)



Cat No : 13967-1-AP

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IFT88, TPR repeat protein 10, TTC10


Positive WB detected inHEK-293 cells, NIH/3T3 cell, MDCK cells, mouse thymus tissue
Positive IP detected inknockout cells and WT cells, HEK-293 cells
Positive IHC detected inhuman heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells, C2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.


Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


13967-1-AP targets IFT88 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, chicken, zebrafish, pig, canine
Immunogen IFT88 fusion protein Ag4980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Synonyms IFT88, TPR repeat protein 10, TTC10
Calculated Molecular Weight 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.


Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-APDownload protocol
IHC protocol for IFT88 antibody 13967-1-APDownload protocol
IF protocol for IFT88 antibody 13967-1-APDownload protocol
IP protocol for IFT88 antibody 13967-1-APDownload protocol
Standard Protocols
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Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.

Authors - Firestone Ari J AJ


Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O


Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.

Authors - Raman M Das

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR

Nat Genet

Complex interactions between genes controlling trafficking in primary cilia.

Authors - Ocbina Polloneal Jymmiel R PJ

Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin