- Featured Product
- KD/KO Validated
IFT74 Polyclonal antibody
IFT74 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HEK-293 cells, Jurkat cells, mouse thymus tissue, Raji cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 27334-1-AP is IFT74 Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA |
| Cited Applications | WB, IP, IF, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | IFT74 fusion protein Ag26385 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | intraflagellar transport 74 homolog (Chlamydomonas) |
| Synonyms | CCDC2, CMG 1, CMG1, IFT74 |
| Calculated Molecular Weight | 600 aa, 69 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC107742 |
| Gene Symbol | IFT74 |
| Gene ID (NCBI) | 80173 |
| RRID | AB_2880847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96LB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT74 antibody 27334-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genet Med Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.
| ||
Pulm Circ Endothelial β‐arrestins regulate mechanotransduction by the type II bone morphogenetic protein receptor in primary cilia | ||
Hum Reprod Biallelic variants in IQCN cause sperm flagellar assembly defects and male infertility | ||
Hum Mol Genet Defective airway intraflagellar transport underlies a combined motile and primary ciliopathy syndrome caused by IFT74 mutations | ||
EMBO J The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport
| ||
EMBO Mol Med IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect |
