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验证数据展示

  • WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

    WB analysis of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

    WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

    IP experiment of HEK-293 using 11083-1-AP (same clone as 11083-1-PBS)

    IP result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

    IHC staining of human prostate cancer using 11083-1-AP (same clone as 11083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

  • WB analysis using 11083-1-AP (same clone as 11083-1-PBS)

    WB analysis using 11083-1-AP (same clone as 11083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 11083-1-AP (same clone as 11083-1-PBS)

    IF Staining of hTERT-RPE1 using 11083-1-AP (same clone as 11083-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT57 antibody (11083-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 11083-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

IFT57 Polyclonal antibody, PBS Only

IFT57 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11083-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Dermal papilla-derived protein 8, DERP8, ESRRBL1, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11083-1-PBS targets IFT57 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag1566

Product name: Recombinant human IFT57 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-429 aa of BC011899

Sequence: MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 57 homolog (Chlamydomonas)
别名 Dermal papilla-derived protein 8, DERP8, ESRRBL1, Estrogen-related receptor beta-like protein 1, HIP1-interacting protein
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC011899
基因名称 IFT57
Gene ID (NCBI) 55081
RRIDAB_2121957
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWB7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)

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