验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells |
| Positive IP detected in | mouse testis tissue, HeLa cells |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17534-1-AP targets IFT52 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse, rat, chicken |
| 免疫原 |
CatNo: Ag11586 Product name: Recombinant human IFT52 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-437 aa of BC039831 Sequence: MEKELRSTILFNAYKKEIFTTNNGYKSMQKKLRSNWKIQSLKDEITSEKLNGVKLWITAGPREKFTAAEFEILKKYLDTGGDVFVMLGEGGESRFDTNINFLLEEYGIMVNNDAVVRNVYHKYFHPKEALVSSGVLNREISRAAGKAVPGIIDEESSGNNAQALTFVYPFGATLSVMKPAVAVLSTGSVCFPLNRPILAFYHSKNQGGKLAVLGSCHMFSDQYLDKEENSKIMDVVFQWLTTGDIHLNQIDAEDPEISDYMMLPYTATLSKRNRECLQESDEIPRDFTTLFDLSIFQLDTTSFHSVIEAHEQLNVKHEPLQLIQPQFETPLPTLQPAVFPPSFRELPPPPLELFDLDETFSSEKARLAQITNKCTEEDLEFYVRKCGDILGVTSKLPKDQQDAKHILEHVFFQVVEFKKLNQEHDIDTSETAFQNNF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intraflagellar transport 52 homolog (Chlamydomonas) |
| 别名 | NGD5, IFT, CGI-53, C20orf9 |
| 计算分子量 | 437 aa, 50 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC039831 |
| 基因名称 | IFT52 |
| Gene ID (NCBI) | 51098 |
| RRID | AB_2280005 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y366 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for IFT52 antibody 17534-1-AP | Download protocol |
| IP protocol for IFT52 antibody 17534-1-AP | Download protocol |
| WB protocol for IFT52 antibody 17534-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. | ||
Am J Hum Genet Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice. | ||
Am J Hum Genet Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
Proc Natl Acad Sci U S A FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia. |
