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验证数据展示

  • WB analysis of HEK-293 using 13615-1-AP

    WB analysis of HEK-293 using 13615-1-AP

    WB result of IFT20 antibody (13615-1-AP, 1:500) with si-Control and si-IFT20 transfected HEK293 cells.

  • IF Staining of hTERT-RPE1 cells using 13615-1-AP

    IF Staining of hTERT-RPE1 cells using 13615-1-AP

    IF result (localization to Golgi-like structures) of anti-IFT20 (13615-1-AP, 1:50) with serum-starved hTERT-RPE1 (MoTH fixed) by Dr. Moshe Kim.

  • WB analysis using 13615-1-AP

    WB analysis using 13615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13615-1-AP

    IP experiment of mouse testis using 13615-1-AP

    IP result of anti-IFT20 (IP:13615-1-AP, 3ug; Detection:13615-1-AP 1:700) with mouse testis tissue lysate 4000ug.

  • IHC staining of human endometrial cancer using 13615-1-AP

    IHC staining of human endometrial cancer using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of human endometrial cancer using 13615-1-AP

    IHC staining of human endometrial cancer using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 13615-1-AP (IFT20 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with IFT20 antibody included
  • WB analysis using 13615-1-AP

    WB analysis using 13615-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13615-1-AP (IFT20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 13615-1-AP

    IHC staining of mouse brain using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of mouse brain using 13615-1-AP

    IHC staining of mouse brain using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of mouse brain using 13615-1-AP

    IHC staining of mouse brain using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of mouse brain using 13615-1-AP

    IHC staining of mouse brain using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of human ovary cancer using 13615-1-AP

    IHC staining of human ovary cancer using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of human placenta using 13615-1-AP

    IHC staining of human placenta using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of mouse ovary using 13615-1-AP

    IHC staining of mouse ovary using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of rat brain using 13615-1-AP

    IHC staining of rat brain using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IHC staining of rat ovary using 13615-1-AP

    IHC staining of rat ovary using 13615-1-AP

    Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 13615-1-AP (IFT20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFT20 antibody included
  • IF Staining of hTERT-RPE1 using 13615-1-AP

    IF Staining of hTERT-RPE1 using 13615-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using IFT20 antibody (13615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

  • Featured Product
  • KD/KO Validated

IFT20 Polyclonal antibody

IFT20 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13615-1-AP

产品说明书

引用文献(90)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

hIFT20, Intraflagellar transport protein 20 homolog

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MDCK cells, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse brain tissue, rat ovary tissue, human placenta tissue, mouse ovary tissue, human ovary cancer tissue, human endometrial cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells
Planning an IHC experiment? We recommend our IHCeasy IFT20 Ready-To-Use IHC Kit. IFT20 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13615-1-AP targets IFT20 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse, rat, canine, monkey, zebrafish
免疫原

CatNo: Ag4521

Product name: Recombinant human IFT20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC038094

Sequence: MAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIFQK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 20 homolog (Chlamydomonas)
别名 hIFT20, Intraflagellar transport protein 20 homolog
计算分子量 15 kDa
观测分子量 15-18 kDa
GenBank蛋白编号BC038094
基因名称 IFT20
Gene ID (NCBI) 90410
RRIDAB_2280001
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium. IFT particles are multi-subunit complexes that are made up of complex A and complex B. IFT20 is a component of IFT complex B and involved in ciliary process assembly. It is associated with the Golgi complex and plays a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium.

实验方案

Product Specific Protocols
IF protocol for IFT20 antibody 13615-1-APDownload protocol
IHC protocol for IFT20 antibody 13615-1-APDownload protocol
IP protocol for IFT20 antibody 13615-1-APDownload protocol
WB protocol for IFT20 antibody 13615-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O
View Article
mouseWB

Nat Aging

A primary cilia-autophagy axis in hippocampal neurons is essential to maintain cognitive resilience

Authors - Manon Rivagorda
  • KD Validated
View Article
mouseIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
View Article
humanIF

Nat Cell Biol

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Authors - Quanlong Lu
View Article
humanIF

Nat Commun

A mutation in VPS15 (PIK3R4) causes a ciliopathy and affects IFT20 release from the cis-Golgi.

Authors - Corinne Stoetzel
View Article
humanWB

Nat Commun

EGF receptor kinase suppresses ciliogenesis through activation of USP8 deubiquitinase.

Authors - Kousuke Kasahara
View Article
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