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验证数据展示

  • WB analysis using 28441-1-AP

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of ARPE-19 using 28441-1-AP

    IF Staining of ARPE-19 using 28441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using IFT172 antibody (28441-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IFT172 Polyclonal antibody

IFT172 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, human

应用

WB, IHC, IF/ICC, ELISA

别名

DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inARPE-19 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28441-1-AP targets IFT172 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat, human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat, human
免疫原 IFT172 fusion protein Ag28921 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 172 homolog (Chlamydomonas)
别名 DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim
计算分子量 1749 aa, 198 kDa
观测分子量 180 kDa
GenBank蛋白编号BC137126
基因名称 IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UG01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFT172 antibody 28441-1-APDownload protocol
IHC protocol for IFT172 antibody 28441-1-APDownload protocol
IF protocol for IFT172 antibody 28441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols