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验证数据展示

  • IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 26952-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26952-1-AP (IFRD2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HL-60 using 26952-1-AP

    WB analysis of HL-60 using 26952-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26952-1-AP (IFRD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IFRD2 Polyclonal antibody

IFRD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26952-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

Interferon-related developmental regulator 2, IFNRP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26952-1-AP targets IFRD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性bovine
免疫原

CatNo: Ag25057

Product name: Recombinant human IFRD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 413-442 aa of BC007265

Sequence: RFEKHLYNAAAFKARTKARSRVRDKRADIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon-related developmental regulator 2
别名 Interferon-related developmental regulator 2, IFNRP
计算分子量 48 kDa
观测分子量 55 kDa
GenBank蛋白编号BC007265
基因名称 IFRD2
Gene ID (NCBI) 7866
RRIDAB_2880700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12894
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFRD2, or interferon-related developmental regulator 2, is a protein that has been identified as a novel factor capable of translationally inactivating ribosomes. It plays a role in regulating gene expression by directly binding to 80S ribosomes, which precludes the binding of mRNA and P-site tRNA, thereby inhibiting translation

实验方案

Product Specific Protocols
WB protocol for IFRD2 antibody 26952-1-APDownload protocol
IHC protocol for IFRD2 antibody 26952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB,IF

J Muscle Res Cell Motil

IFRD2, a target of miR-2400, regulates myogenic differentiation of bovine skeletal muscle satellite cells via decreased phosphorylation of ERK1/2 proteins

Authors - Zhian Gong
View Article