验证数据展示
经过测试的应用
| Positive WB detected in | EL-4 cells |
| Positive IP detected in | EL-4 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31951-1-AP targets IFNGR1/CD119 in WB, IP, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg1536 Product name: Recombinant Mouse IFN-gamma R1/IFNGR1 protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 26-254 aa of NM_010511.3 Sequence: ALTSTEDPEPPSVPVPTNVLIKSYNLNPVVCWEYQNMSQTPIFTVQVKVYSGSWTDSCTNISDHCCNIYEQIMYPDVSAWARVKAKVGQKESDYARSKEFLMCLKGKVGPPGLEIRRKKEEQLSVLVFHPEVVVNGESQGTMFGDGSTCYTFDYTVYVEHNRSGEILHTKHTVEKEECNETLCELNISVSTLDSRYCISVDGISSFWQVRTEKSKDVCIPPFHDDRKDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | interferon gamma receptor 1 |
| 别名 | CD119, IFN-gamma R1, IFN-gamma receptor 1, IFN-gamma-R1, IFN-gamma-R-alpha |
| 计算分子量 | 52kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | NM_010511.3 |
| 基因名称 | Ifngr1 |
| Gene ID (NCBI) | 15979 |
| RRID | AB_3670157 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P15261 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for IFNGR1/CD119 antibody 31951-1-AP | Download protocol |
| WB protocol for IFNGR1/CD119 antibody 31951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
