• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 10522-1-AP

    WB analysis using 10522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10522-1-AP (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 10522-1-AP

    FC experiment of MCF-7 using 10522-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug IFNAR2 Polyclonal antibody (10522-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

IFNAR2 Polyclonal antibody

IFNAR2 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 10522-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, FC (Intra), ELISA and More (2)

别名

IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10522-1-AP targets IFNAR2 in WB, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human
文献引用反应性human, pig
免疫原 IFNAR2 fusion protein Ag0191 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon (alpha, beta and omega) receptor 2
别名 IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2
计算分子量 331 aa, 37 kDa
观测分子量 58-70 kDa
GenBank蛋白编号BC002793
基因名称 IFNAR2
Gene ID (NCBI) 3455
RRIDAB_10694421
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48551
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFNAR2 antibody 10522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA

Authors - Yong Ge
View Article
humanWB,IP

J Virol

African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway

Authors - Guangqiang Ye
View Article
humanWB

J Virol

Herpes simplex virus 1 UL36USP antagonizes type I IFN-mediated antiviral innate immunity.

Authors - Hui Yuan
View Article
humanWB,IP,IF

J Biol Chem

Protein kinase A suppresses anti-proliferative effect of interferon-α in hepatocellular carcinoma by activation of protein tyrosine phosphatase SHP2

Authors - Yuwen Sheng
View Article
pigWB

PLoS Pathog

African swine fever virus pB318L, a trans-geranylgeranyl-diphosphate synthase, negatively regulates cGAS-STING and IFNAR-JAK-STAT signaling pathways

Authors - Xiaohong Liu
View Article