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IFN gamma Polyclonal antibody

IFN gamma Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29788-1-AP

Print datasheet

Synonyms

Ifg, IFN g, IFN gamma, Ifng, interferon gamma

验证数据展示

  • WB analysis using 29788-1-AP

    WB analysis using 29788-1-AP

    PMA, Ionomycin and Brefeldin A treated CTLL-2 cells were subjected to SDS PAGE followed by western blot with 29788-1-AP (IFN gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPMA, Ionomycin and Brefeldin A treated CTLL-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29788-1-AP targets IFN gamma in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA
Tested Reactivity mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon gamma
Synonyms Ifg, IFN g, IFN gamma, Ifng, interferon gamma
Calculated Molecular Weight17 kDa
Observed Molecular Weight15-18 kDa
GenBank Accession NumberBC119063
Gene Symbol Ifng
Gene ID (NCBI) 15978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01580
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFN gamma (IFNG) is a soluble cytokine that is the only member of the type II class of IFN. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB).

实验方案

Product Specific Protocols
WB protocol for IFN gamma antibody 29788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols