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IFN-alpha Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat no : MP03090-1

Synonyms

IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03090-1, Human IFN-alpha Recombinant Matched Antibody Pair - PBS only. 87610-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35032. 87610-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

    Sandwich ELISA standard curve of MP03090-1

    Sandwich ELISA standard curve of MP03090-1, Human IFN-alpha Recombinant Matched Antibody Pair - PBS only. 87610-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35032. 87610-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Plasma of seven individual healthy human donors was measured. The human IFN-alpha concentration of detected samples was determined to be 24.69 pg/mL with a range of 13.9 - 42.9 pg/mL.

    Sandwich ELISA sample test of MP03090-1

    Plasma of seven individual healthy human donors was measured. The human IFN-alpha concentration of detected samples was determined to be 24.69 pg/mL with a range of 13.9 - 42.9 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IFN-alpha concentration was determined to be 104.4 pg/mL in unstimulated PBMC supernatant, 325.9 pg/mL in PHA stimulated PBMC supernatant.

    Sandwich ELISA sample test of MP03090-1

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IFN-alpha concentration was determined to be 104.4 pg/mL in unstimulated PBMC supernatant, 325.9 pg/mL in PHA stimulated PBMC supernatant.

  • The mean IFN-alpha concentration was determined to be 38.48 ng/mL in HEK-293T cell extract based on a 1.3 mg/mL extract load.

    Sandwich ELISA sample test of MP03090-1

    The mean IFN-alpha concentration was determined to be 38.48 ng/mL in HEK-293T cell extract based on a 1.3 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP03090-1 targets IFN-alpha in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: IFN-alpha Recombinant monoclonal antibody, PBS Only (Capture) 87610-2-PBS (252039D11). 100 μg. Concentration 1 mg/ml.

Detection antibody: IFN-alpha Recombinant monoclonal antibody, PBS Only (Detector) 87610-1-PBS (252039B7). 100 μg. Concentration 1 mg/ml.

Matched pair protein standard: Eg2592

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 87610-2-PBS
Tested Reactivity human
Immunogen IFN-alpha fusion protein Eg2592
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IFNA1
Synonyms IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1
GenBank accession numberNM_024013.2
Gene symbol IFN alpha1
UniprotP01562
Gene ID (NCBI) 3439
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 87610-1-PBS
Tested Reactivity human
Immunogen IFN-alpha fusion protein Eg2592
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IFNA1
Synonyms IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1
GenBank accession numberNM_024013.2
Gene symbol IFN alpha1
UniprotP01562
Gene ID (NCBI) 3439
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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