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验证数据展示

  • WB analysis of Recombinant protein using 87610-3-RR

    WB analysis of Recombinant protein using 87610-3-RR

    Human IFN-alpha recombinant protein was subjected to SDS PAGE followed by western blot with 87610-3-RR (IFNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 87610-3-RR

    WB analysis using 87610-3-RR

    CHO cells transfected with human IFN-alpha were subjected to SDS PAGE followed by western blot with 87610-3-RR (IFNA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of Transfected CHO using 87610-3-RR

    IF Staining of Transfected CHO using 87610-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO using IFN-alpha antibody (87610-3-RR, Clone: 252039C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity of 87610-3-RR

    Affinity of 87610-3-RR

    Biolayer interferometry (BLI) kinetic assays of 87610-3-RR against Human IFN-alpha were performed. The affinity constant is 0.989 nM.

IFN-alpha Recombinant monoclonal antibody

IFN-alpha Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 87610-3-RR

产品说明书

CloneNo. 252039C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRecombinant protein, Transfected with IFNA1 CHO cells
Positive IF/ICC detected inTransfected CHO

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87610-3-RR targets IFN-alpha in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2592

Product name: Recombinant Human IFNA1 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-189 aa of NM_024013.2

Sequence: CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQLNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IFNA1
别名 IFN ALPHA, IFNA1, IFN alpha 1/13, IFN alpha1, IFNA 1
计算分子量22 kDa
GenBank蛋白编号NM_024013.2
基因名称 IFN alpha1
Gene ID (NCBI) 3439
RRIDAB_3745647
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01562
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFNA1 (IF-alpha) is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). IFNA1 induces B cell maturation into plasma cells and immunoglobulin production. IFNA1 plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). IFNA1 was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.

实验方案

Product Specific Protocols
IF protocol for IFN-alpha antibody 87610-3-RRDownload protocol
WB protocol for IFN-alpha antibody 87610-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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