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验证数据展示

  • WB analysis using 83455-5-RR (same clone as 83455-5-PBS)

    WB analysis using 83455-5-RR (same clone as 83455-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83455-5-RR (IFITM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83455-5-RR (same clone as 83455-5-PBS)

    FC experiment of MCF-7 using 83455-5-RR (same clone as 83455-5-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM2 Recombinant antibody (83455-5-RR, Clone:240318B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    WB analysis of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    WB result of IFITM2 antibody (83455-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFITM2 transfected HepG2 cells. This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    IF Staining of HepG2 using 83455-5-RR (same clone as 83455-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83455-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83455-5-RR

    Affinity and Kinetic Characterization of 83455-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83455-5-RR against Human IFITM2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

IFITM2 Recombinant monoclonal antibody, PBS Only

IFITM2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83455-5-PBS

产品说明书

CloneNo. 240318B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

1 8D, 18D, 240318B6, DSPA2c, IFITM 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy IFITM2 Ready-To-Use IHC Kit. IFITM2 primary antibody included.

产品信息

83455-5-PBS targets IFITM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3451

Product name: Recombinant human IFITM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC009696

Sequence: MNHIVQTFSPVNSGQPPNYEMLKEEQEVAMLGAPHNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNTCCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKCLNIWALILGIFMTILLVIIPVLVVQAQR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon induced transmembrane protein 2 (1-8D)
别名 1 8D, 18D, 240318B6, DSPA2c, IFITM 2
计算分子量 132 aa, 15 kDa
观测分子量 15 kDa
GenBank蛋白编号BC009696
基因名称 IFITM2
Gene ID (NCBI) 10581
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ01629
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

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