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IFITM2 Polyclonal antibody
IFITM2 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | MCF-7 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12769-1-AP targets IFITM2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | FC, IF, IHC, IP, WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Immunogen | IFITM2 fusion protein Ag3451 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interferon induced transmembrane protein 2 (1-8D) |
| Synonyms | 1 8D, IFITM2 |
| Calculated Molecular Weight | 132 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC009696 |
| Gene Symbol | IFITM2 |
| Gene ID (NCBI) | 10581 |
| RRID | AB_2122089 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. Catalog#12769-1-AP is a rabbit polyclonal antibody produced with full-length of human IFITM2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM2 antibody 12769-1-AP | Download protocol |
| IHC protocol for IFITM2 antibody 12769-1-AP | Download protocol |
| IF protocol for IFITM2 antibody 12769-1-AP | Download protocol |
| IP protocol for IFITM2 antibody 12769-1-AP | Download protocol |
| FC protocol for IFITM2 antibody 12769-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
| ||
Cell Host Microbe IFITM Proteins Incorporated into HIV-1 Virions Impair Viral Fusion and Spread.
| ||
Nat Commun IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses | ||
Nat Commun BAG3 promotes pancreatic ductal adenocarcinoma growth by activating stromal macrophages.
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