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IFIT2 Polyclonal antibody

IFIT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12604-1-AP

Print datasheet

Synonyms

cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54



经过测试的应用

Positive WB detected inA431 cells, RAW 264.7 cells, THP-1 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman skin cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12604-1-AP targets IFIT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen IFIT2 fusion protein Ag3283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IFIT 2
Synonyms cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54
Calculated Molecular Weight 484 aa, 56 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC032839
Gene Symbol IFIT2
Gene ID (NCBI) 3433
RRIDAB_2864734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09913
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

实验方案

Product Specific Protocols
WB protocol for IFIT2 antibody 12604-1-APDownload protocol
IHC protocol for IFIT2 antibody 12604-1-APDownload protocol
IF protocol for IFIT2 antibody 12604-1-APDownload protocol
IP protocol for IFIT2 antibody 12604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB

Nat Immunol

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.

Authors - Ying Kai Chan
humanWB

Mol Cell

Translation stress and collided ribosomes are co-activators of cGAS.

Authors - Li Wan
humanWB

Nat Microbiol

ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.

Authors - GuanQun Liu
humanWB

Nat Microbiol

Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.

Authors - Vy Tran

Nucleic Acids Res

IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.

Authors - Renata C Fleith