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验证数据展示

  • IHC staining of mouse testis using 21483-1-AP

    IHC staining of mouse testis using 21483-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21483-1-AP (IFIT1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of RAW 264.7 using 21483-1-AP

    WB analysis of RAW 264.7 using 21483-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 21483-1-AP (IFIT1L Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HEK-293 using 21483-1-AP

    IF Staining of HEK-293 using 21483-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21483-1-AP (IFIT1L antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

IFIT1L Polyclonal antibody

IFIT1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21483-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

IFIT1B, IFIT1L

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IHC detected inmouse testis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21483-1-AP targets IFIT1L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat, mouse
免疫原

CatNo: Ag15765

Product name: Recombinant human IFIT1L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 302-474 aa of BC150189

Sequence: IKEATNWQPRGQDRETVDRLVQLAICKFEKTIMLKRTFEMAYVDLAETYAEIGHHRKAEEHFQKGLRMKIFEDQLKQEIHYHYGRFQEHHGKSQDKAITHYLKGLKIEKMSHSREKLLNALEKLAKRCIHQNVRVVESVSLLGLIHKLKGEVSDALLCYERALRLAADLNPIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon-induced protein with tetratricopeptide repeats 1-like
别名 IFIT1B, IFIT1L
计算分子量 474 aa, 55 kDa
观测分子量 55-65 kDa
GenBank蛋白编号BC150189
基因名称 IFIT1L
Gene ID (NCBI) 439996
RRIDAB_2878867
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T764
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for IFIT1L antibody 21483-1-APDownload protocol
IHC protocol for IFIT1L antibody 21483-1-APDownload protocol
WB protocol for IFIT1L antibody 21483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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